Despite the central role of Pdx PDX in

2018-10-20

Despite the central role of Pdx1/PDX1 in orchestrating pancreatic morphogenesis in mice and humans, vanishingly little is known about its direct transcriptional targets. Here, we chose to address this deficit by developing an in vitro human embryonic stem cell (hESC) differentiation protocol that sp

br Author contributions br Resource table Resource details P

2018-10-20

Author contributions Resource table. Resource details Previously we have generated an induced pluripotent stem cell line (named MHHi002-A) from a male CF patient homozygous for the mutation p.Phe508del in the CFTR gene. Thereby the absence of the three bases CTT results in the deletion of phe

From previous studies we know that cells above the bulge

2018-10-20

From previous studies we know that cells above the bulge that are Sca-1 and CD34 negative also possess stem cell properties and that these stem cells are not label retaining cells, but relatively active in p-selectin (Jensen et al., 2008). This suggests that LRCs primarily reflects a certain patter

Embryonic stem cell protocols frequently employ irradiated M

2018-10-20

Embryonic stem cell protocols frequently employ irradiated MEFs as feeder cultures (Conner and fibroblast, 2001). We plated mouse embryonic stem (ES) methysergide either with or without feeders and compared ALP expression in both cultures using our multiplexed luminescence method. Higher levels of

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2018-10-20

Experimental Procedures Author Contributions Acknowledgments Introduction Human embryonic stem cells (hESCs) are capable of unlimited self-renewal in culture while maintaining the potential to differentiate into any cell type present in the human body and thus provide researchers great o

br Experimental Procedures br Author Contributions br

2018-10-20

Experimental Procedures Author Contributions Acknowledgments This work was supported by grants from the Ministerio de Ciencia e Innovación (Grant SAF2012-38914), Plan Nacional de I + D + I, Spain and the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR) (Grant 2014 SGR229), Depar

It has previously been reported that differentiation of hESC

2018-10-20

It has previously been reported that differentiation of hESCs without the embryonic body stage led to an increase in the capacity of hESCs to differentiate into osteogenic progenitor c75 (Karp et al., 2006). Consistent with these findings, our differentiation culture method resulted in cells with i

To gain further insight into progenitor cells

2018-10-20

To gain further insight into progenitor gtpase derived from human skeletal muscle, we performed comprehensive cell-surface protein profiling of two progenitor populations, CD56+ myogenic progenitors and PDGFRα+ mesenchymal progenitors. This comprehensive analysis identified previously unrecognized m

IGF and PI K signaling

2018-10-20

IGF and PI3K signaling in regenerating muscle was tightly regulated across multiple levels and temporally, especially during the transition between myoblast proliferation and differentiation. In the early period, IGF1 was upregulated and quickly followed by increases in expression and chromatin remo

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2018-07-29